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The expression of CD36 on macrophages post different treatments was also evaluated by immunofluorescence staining using anti-CD36 antibody (incubated overnight at 4 C) and Alexa Fluor 488-conjugated secondary antibody (incubated for 1 h at room temperature)

The result could not be fully confirmed as there are some limitations

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18 Available from: 19 LeteurtreSMartinotADuhamelAProulxFGrandbastienBCottingJet alValidation of the paediatric logistic organ dysfunction (PELOD) score: prospective, observational, multicentre study

Pharmacological inhibition of the chemokine CCL2 (MCP-1) diminishes liver macrophage infiltration and steatohepatitis in chronic hepatic injury